| 摘要: |
| 目的建立L2.3神经干细胞糖氧剥夺/再灌注损伤模型,探讨糖氧剥夺/再灌注是否可以抑制L2.3神经干细胞N-Myc-DLL3-Notch信号系统。方法体外培养的L2.3神经干细胞经历糖氧剥夺(OGD)6h,再灌注(R)16h,建立OGD/R模型。分别在OGD前、OGD6h、OGD6h再灌注16h,用western blot方法检测Notch1、NICD、DLL3和N-Myc蛋白表达情况。结果与糖氧剥夺/再灌注前比,糖氧剥夺6h,再灌注16h可下调Notch1、NICD、DLL3和N-Myc蛋白表达。结论糖氧剥夺/再灌注损伤可抑制L2.3神经干细胞N-Myc-DLL3-Notch信号系统 |
| 关键词: 糖氧剥夺/再灌注 L2.3细胞 Notch1 DLL3 N-Myc |
| DOI： |
| 分类号:R329.24； R654.1； R543 |
| 基金项目:四川省科技厅应用基础研究项目资助(编号:2015JY0064);四川省科技厅支撑计划项目(编号:2015FZ0075) |
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| Glucose Deprivation/Reperfusion Inhibits N-Myc-DLL3-Notch Signaling System of L2.3 Neural Stemcells |
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Wu Lan;Jiang Xiaoqin;Zhou Wenqin
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West China second Hospital of Sichuan University Chengdu
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| Abstract: |
| Objective To establish L2.3 neural stem cells model of glucose and oxygen deprivation/reperfusion injury,and to investigate whether glucose deprivation/reperfusion can inhibit N-Myc-DLL3-Notch signaling system of L2.3 neural stem cells.Methods L2. 3 neural stem cells in vitro cultured were given oxygen glucose deprivation( OGD) 6h and reperfusion( R) 16 h,and then a OGD/R model was established.The expression of Notch1、NICD、DLL3 and N-Myc protein was detected by western blot method at before OGD,OGD6h、16h after reperfusion,respectively.Results Compared with before oxygen and glucose deprivation/reperfusion,the expression of Notch1、NICD、DLL3 and N-Myc protein decreased after 6h of glucose and oxygen and glucose deprivation and 16h of reperfusion. Conclusion Oxygen and glucose deprivation/reperfusion injury can inhibit N-MycDLL3-Notch signaling system of L2.3 neural stem cells |
| Key words: Glucosedeprivation/reperfusion L2.3cells Notch1 DLL3 N-Myc |
